http://www.polyploidevolution.com/resources daily 0.75 2014-12-07 http://www.polyploidevolution.com/travel daily 0.75 2014-12-07 http://www.polyploidevolution.com/work daily 1.0 2013-08-30 http://www.polyploidevolution.com/teaching2 daily 0.75 2017-01-11 https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a825e4e4b0e845493c5853/54ac1743e4b04f1bf4db66a6/1420565513115/IMG_0129.JPG Teaching - Laboratory in Ecology & Evolution Students examine insects collected during a laboratory exercise focused on generatine species accumulation curves. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a825e4e4b0e845493c5853/54ac1748e4b0b365f77e84d3/1420565291305/WaterLab1.JPG Teaching - Laboratory in Ecology & Evolution Students sample a local waterway, obtaining subsurface fractions to assay several characteristics. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a825e4e4b0e845493c5853/54ac1749e4b0d6033e58687e/1420565193006/IMG_6717.JPG Teaching - Laboratory in Ecology & Evolution Students work to determine the soil type and composition of samples taken from old and secondary-forest plots near the University of Rochester campus. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a825e4e4b0e845493c5853/54ac1746e4b0b365f77e84be/1420565073808/IMG_0284.JPG Teaching - Local fauna studies around campus Students prepared and distributed these artificial shelters made of plywood throughout old and secondary-growth forest fragments near the University of Rochester campus. This allowed them to create species inventories for local amphibians, reptiles and invertibrates. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a825e4e4b0e845493c5853/54ac1744e4b041b86b091ab5/1420564812203/IMG_0248.JPG Teaching - Laboratory in Ecology & Evolution A student loads a gel with PCR product as she accumulates data for her final, independently designed research project. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a825e4e4b0e845493c5853/54ac1744e4b0da465dd2e66b/1420564752613/IMG_0194.JPG Teaching - Laboratory in Ecology & Evolution Students assay for several characteristics of a local waterway in Rochester, NY. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a825e4e4b0e845493c5853/54ac1742e4b0da465dd2e664/1420564611265/IMG_0047.JPG Teaching - Laboratory in Ecology & Evolution Students practice laying out a grid to prepare to count local herbs for a laboratory exercise focused on sampling methods and microhabitats. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a825e4e4b0e845493c5853/54ac1742e4b0b365f77e849d/1420564627488/IMG_0040.JPG Teaching - Laboratory in Ecology & Evolution Students count herbs in a quadrat frame during a laboratory exercise focused on sampling methods and microhabitats. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a825e4e4b0e845493c5853/54a825eee4b0e845493c588c/1420307617124/Woodlands+boards+Ariel+Adam_5311.JPG Teaching - Preparing shelter boards for reptile/amphibian survey In addition to an extensive floristic survey in old-growth and secondary-growth forests around the University of Rochester, students were also interested in describing the reptiles and amphibians in those area. Sheets of plywood were prepared and distributed in these areas. Students could then return and lift the shelters to see what animals were present in these areas. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a825e4e4b0e845493c5853/54a825ece4b037e9bac8c9ad/1420307200140/Platform+crew_0939.JPG Teaching - The 'Platform crew' celebrates The 'platform crew' celebrates finishing the installation of the last series of platforms. These were placed in low-lying areas on trails connecting university housing to the main part of the University of Rochester campus through an area of old-growth forest. The platforms allowed for increased foot traffic in muddy conditions while also preventing degredation of the trails. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a825e4e4b0e845493c5853/54a825ebe4b0e845493c5888/1420306941601/Irondequoit+Bio264+sassafras+hands_6431.JPG Teaching - What's your favorite Sassafrass leaf morphotype? The field crew votes with their hands on their favorite leaf shape on the Sassafrass tree. It looks like the 'mitten' shape wins! https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a825e4e4b0e845493c5853/54a825e8e4b0e845493c5871/1420306806409/IMG_5590+-+Copy.jpg Teaching - The forest survey gang Laney, Maria, Annalise and Layla show off their intimidating skill sets as they survey forest and soil composition in natural areas around Rochester, NY. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a825e4e4b0e845493c5853/54a825e8e4b037e9bac8c992/1420306500138/IMG_1865.JPG Teaching - Wood stake luau? Annalise and Dana get creative while building 'stake mats' I designed to reduce degradation of trails through primary forest areas near the University of Rochester Campus. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a825e4e4b0e845493c5853/54a825e8e4b0e845493c586f/1420307612268/IMG_0451.JPG Teaching - Experimental garden The field crew (and Heisey) take a break from working to set up an extensive experimental garden near the University of Rochester Campus. The garden was home to several multi-season experiments involving ivy and yarrow. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a825e4e4b0e845493c5853/54a825e7e4b037e9bac8c980/1420307603569/IMG_0231.JPG Teaching - Invasive plant removal Part of the field crew (Little Adam, Caitlin and Colleen) work to remove invasive plants from a power-line corridor near the University of Rochester Campus. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a825e4e4b0e845493c5853/54a825e5e4b0e845493c5858/1420306027026/IMG_0149.JPG Teaching - Poison Ivy! Little Justin and I take some official 'boot' measurements of a large poison ivy vine in Rochester, NY. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a84988e4b07985e37012e0/54a84a21e4b0e845493cfccc/1420315710705/Prizes.JPG Teaching - Prize winners Allison (best proposal presentation), James (best written proposal) and Scott (most creative proposal) show off their prizes. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a84988e4b07985e37012e0/54a84998e4b07985e370133f/1420315887066/Kelly.JPG Teaching - Grant proposal presentation Kelly presents her grant proposal project entitled: Measuring current population viability across Gray Wolf (Canis lupus) populations and the benefits of reintroducing gene flow via genetic rescue and habitat corridors. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a84988e4b07985e37012e0/54a84998e4b00bf98ff2ee5f/1420315622589/Eric.JPG Teaching - Grant proposal presentation Eric presents his grant proposal project entitled: A phylogeographic analysis of a plant-insect interaction in the Australian Alps, Victoria Australia. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a84988e4b07985e37012e0/54a84989e4b00bf98ff2ee22/1420315302388/Skull2.JPG Teaching - 3D printed Australopithecus africanus Used for a lesson on human evolution, this 3D printed skull was created from a scan of the original 'Taung child' find - a ~2+ million year old skull of a human ancestor (Australopithecus africanus) found in a South African cave in 1924. Radiolab did a great 20 min podcast on the discovery and provided the Makerbot file: http://www.radiolab.org/story/taung-child/ https://static1.squarespace.com/static/548475cde4b0982d899e8797/t/5875c0eb5016e196ea726f02/1484112120897/ Teaching https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a835bae4b0f1bacf895d74/54a835c1e4b0d1cd06f1733c/1420310303218/IMG_5278.JPG Teaching - Arabidopsis arrestors Ally works to assemble custom cages I designed to manage the growth of Arabidopsis ecotypes during various experiments. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a835bae4b0f1bacf895d74/54a835bee4b0d1cd06f17325/1420310085226/IMG_5263.JPG Teaching - Collecting data Emily measures several aspects of growth of this ecotype of Arabidopsis thaliana during a large-scale common garden experiment. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a835bae4b0f1bacf895d74/54a835bce4b0f7d742e03902/1420310464495/IMG_5254.JPG Teaching - Plant bagging Kyla slips a plant-isolation bag over a plant during a late stage of this large-scale common garden experiment. https://static1.squarespace.com/static/548475cde4b0982d899e8797/t/5484863ce4b0b436d77ec8e0/1417971266569/P1000156.JPG Teaching https://static1.squarespace.com/static/548475cde4b0982d899e8797/t/5485b984e4b089bd3e5adca5/1418049935258/IMG_5263.JPG Teaching https://static1.squarespace.com/static/548475cde4b0982d899e8797/t/54872184e4b02be55de0832b/1418142087935/Skull2.JPG Teaching https://static1.squarespace.com/static/548475cde4b0982d899e8797/t/56e49c287da24f279613a618/1457822762406/11.16.15-Sci-SS-launch.png Teaching http://www.polyploidevolution.com/research-1 daily 0.75 2016-03-12 https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a877ece4b0d6033e49928e/54ac3568e4b0ab38fef474b6/1420572101365/GOPR9258.JPG Research - Common garden experiment Emily and I discuss the progress of diploid and polyploid Arabidopsis in my large-scale common garden experiment. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a877ece4b0d6033e49928e/54a87e04e4b034981b53dc1b/1420328845801/seedjail2.jpg Research - Creating neopolyploid Arabidopsis Prior to the large-scale common garden experiment, I created neopolyploid lines of each Arabidopsis ecotype. This was carried out through several steps, the first of which is shown here: growing diploid seeds on a sterile growth matrix. Later, these plants will be exposed to a chemical that induces genome duplication and then extensively assayed for total chromosome count. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a877ece4b0d6033e49928e/54a87e04e4b0d132f65957ac/1420329074560/HarvestPicBig.JPG Research - Post-harvest drying After completion of the growth phase of the major common garden experiment, each plant was harvested and allowed to dry, before seed was collected and several morphometric measurements were made. Here, approximately 1/2 of all the plants in this experiement can be seen. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a877ece4b0d6033e49928e/54a877f7e4b0ab38fee4e808/1420327761013/IMG_5256.JPG Research - Arabidopsis arrestors After reaching a certain size, each plant is contained in a custom-built cage (Arabidopsis arrestor). This helps to maintain separation between plants and makes it easier to harvest each individual, collect seed and measure plant traits at the conclusion of the growth phase of the experiment. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a877ece4b0d6033e49928e/54a877f5e4b0ab38fee4e7f2/1420327377366/IMG_4900.JPG Research - Arabidopsis bolt As the plants begin to pass though developmental milestones, they are measured and plant tags are marked. In this case, a blue star means the plant has been recorded as bolting. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a877ece4b0d6033e49928e/54a877f3e4b0ab38fee4e7ef/1420327497307/IMG_4895.JPG Research - Flowing Arabidopsis As each plant becomes mature and begins to flower, additional morphometric measurements are made and developmental time-points are recorded. Here, a gold star has been added to the plant tag, indicating that the flowering date of this plant has been recorded. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a877ece4b0d6033e49928e/54a877f4e4b0ac4256e62ed0/1420327286680/IMG_4879.JPG Research - Arabidopsis rosettes About one week post-planting, morphological differences can already be seen among the different ecotypes and ploidy levels of this experimental Arabidopsis plants. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a877ece4b0d6033e49928e/54a877f1e4b0ab38fee4e7ed/1420572290991/IMG_4877.JPG Research - Arabidopsis rosettes By 10 days post-planting, Arabidopsis has begun to form immature rosettes. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a877ece4b0d6033e49928e/54a877f0e4b0ac4256e62eb9/1420327113845/IMG_4817.JPG Research - Arabidopsis seedlings Five genetically identical seeds were sown into each pot. After a few days, all but one seedling was removed. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a877ece4b0d6033e49928e/54a877eee4b0ac4256e62eb6/1420327892560/IMG_4816.JPG Research - Growth chamber Here, the experimental Arabidopsis ecotypes (both diploids and neopolyploids) begin to germinate. The time-lapse camera that generated the above video can be seen on the far wall. About half of all the plants in this experiment can be seen here. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a85222e4b0ac4256e56d6c/54ac3d7fe4b01ff9ee677ed7/1420574276453/helix+minorMarmorata.jpg Research - Variegated Hedera helix An example of variegated (chimeric, or mottled, expression of chlorophyll) Hedera helix. Notice the trichomes, or leaf hairs, that are barely visible on the central green parts of the leaf. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a85222e4b0ac4256e56d6c/54a854f2e4b04f1bf4cbe8c9/1420322408669/IMG_0051.JPG Research - Harvesting Hedera Undergraduate Laya helps to harvest Hedera cuttings that had been growing for two seasons. After harvesting, each plant was pressed, then disassembled. Each leaf was photographed and several measurements taken for morphometric description. Characteristics of stems and roots were also measured. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a85222e4b0ac4256e56d6c/54a8549ae4b0631d0c4f88b8/1420321299885/Cold+frames+Adam+Justin+friendly_5083.jpg Research - Homemade cold-frames Justin and I setting up cold frames I designed to overwinter hundreds of Hedera plants for a multi-season experiment investigating the morphology and growth potential of various species of ivy. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a85222e4b0ac4256e56d6c/54a85416e4b0d1cd06f20337/1420573811829/nepalensis+sinensis2-66x-use.jpg Research - Scale trichome A scale-type trichome on a Hedera nepalensis leaf. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a85222e4b0ac4256e56d6c/54a85416e4b0970e0f164a4f/1420573807261/maderensis1-use.jpg Research - Scale trichome A scale-type trichome on a Hedera maderensis leaf. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a85222e4b0ac4256e56d6c/54a85416e4b0e845493d2fac/1420573779165/hibernica+Lake94-2-use.jpg Research - Stellate trichome A stellate-type trichome on a Hedera hibernica leaf. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a85222e4b0ac4256e56d6c/54a85416e4b0d1cd06f20333/1420573762285/helix+Tanja3-use.jpg Research - Stellate trichome A stellate-type trichome on a Hedera helix leaf. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a85222e4b0ac4256e56d6c/54a85416e4b03d35accb8ce9/1420655410054/colchica+MyHeart+both5.jpg Research - Mixed trichomes Stellate and scale trichomes co-occurring on Hedera colchica. I discovered this phenomenon, which is not restricted to this species, and has never been reported. This emphasizes the importance of a morphologically-independent method to identify the species of Hedera, especially in areas under invasion. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a85222e4b0ac4256e56d6c/54a85223e4b0631d0c4f7c23/1420318721421/leaf2.jpg Research - Ivy leaf morphology, plate 2 Photos of leaves from various species of Hedera that I grew under common conditions. Ploidy levels within species are stable, but diploid, tetraploid, hexaploid and octoploid species exist within the genus. https://static1.squarespace.com/static/548475cde4b0982d899e8797/54a85222e4b0ac4256e56d6c/54a85223e4b010b9ae2d9cf2/1420324264985/leaf1.jpg Research - Ivy leaf morphology, plate 1 Photos of leaves from various species of Hedera that I grew under common conditions. Ploidy levels within species are stable, but diploid, tetraploid, hexaploid and octoploid species exist within the genus https://static1.squarespace.com/static/548475cde4b0982d899e8797/t/54ac352be4b034981b633ba0/1420571962146/GOPR9258.JPG Research https://static1.squarespace.com/static/548475cde4b0982d899e8797/t/5485ba95e4b07ca5e645a068/1418050199533/OldHomesite_NCcropped.jpg Research https://static1.squarespace.com/static/548475cde4b0982d899e8797/t/5485beb3e4b0a614d0de45d2/1418051251854/Slide2.jpg Research http://www.polyploidevolution.com/service daily 0.75 2016-03-13 http://www.polyploidevolution.com/about daily 0.75 2020-12-23 https://static1.squarespace.com/static/548475cde4b0982d899e8797/t/54847a26e4b0e74f065383e0/1417968168986/ About